Similar to A-23187, 4-Bromo A-23187 is used for in situ calibrations of fluorescent Ca2+ indicators to equilibrate intracellular and extracellular calcium concentrations and to allow Mn2 to enter cells to quench indicator fluorescence. However, since 4-bromo A-23187 is nonfluorescent, it is preferred over the blue fluorescent A-23187 for calibrations of UV-excited Ca2+ indicators such as Indo-1 and Fura-2 in order to keep background fluorescence to a minimum.
- White solid soluble in DMSO or EtOH
- Store at 4°C
- MW: 602.52