5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment. Also see the membrane-permeant compound dichlorofluorescein diacetate (51016).
- pKa = 4.8
- λEx/λEm = 504/529 nm
- λµ= 90,000
- Orange solid soluble in DMSO and pH>6 buffers
- Store at 4°C and protect from light, especially in solution
- MW: 445
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