AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm). The detection range of the assay is 0.1-15 ug/mL protein. AccuOrange is much more sensitive than traditional protein quantitation assays such as BCA, Bradford and Lowry, and shows superior linearity and reproducibility compared to the NanoOrange protein quantitation assay. The assay shows minimal variability between different proteins, and has stable fluorescence signal for up to 16 hours. AccuOrange is recommended for quantitating purified protein or antibody samples. The tolerance of the AccuOrange assay to salts, buffers, detergents, and other chemicals is shown in Table 1 of the product protocol. The AccuOrange assay has low tolerance for non-ionic detergents, and is not recommended for use with cell lysates containing Triton X-100, sodium deoxycholate, CHAPs, or other non-ionic detergents. The assay can tolerate up to 0.01% SDS (final concentration in assay).The AccuOrange Assay is compatible with the AccuLite™ 470 Mini Fluorometer.