Biotin-4-fluorescein (N-(5-aminopentyl)biotinamide, trifluoroacetic acid salt) has been reported to be a superior fluorescent biotin derivative for accurate measurement of avidin and streptavidin in crude biological samples. Binding of biotin to avidin and streptavidin is high affinity, fast and noncooperative.
- λEx/λ Em = 494/523 nm
- Yellow solid soluble in DMSO, or pH>7 buffers
- Store at -20°C and protect from light
- MW: 644.7