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Biotin-rhodamine 110

401.80

Clear
Catalog #: 80022.

Product Description

We developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019),both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples. In addition, biotin-rhodamine 110 can be used as a polar tracer to study the morphology of cells, similar to the use of Lucifer Yellow cadaverine biotin-X (80017). The dye rhodamine 110 (or carboxyrhodamine 110) has absorption and emission wavelengths similar to those of fluorescein. However, the spectra and fluorescent quantum yield of rhodamine 110 are relatively unaffected by pH (pH 4-9), whereas the fluorescence of fluorescein is significantly reduced at acidic pH. Moreover, rhodamine 110 is much more photostable than fluorescein, making biotin-rhodamine 110 a better choice for studies where prolonged exposure to light may be necessary.

  • λExEm (MeOH) = 502/524 nm
  • Orange red solid soluble in DMF or DMSO
  • Store at 4°C and protect from light, especially in solution
  • C41H50N6O9S
  • MW: 802.94

n/a

MSDS (PDF): MSDS 80022

5 mg

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life