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Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

61.601,890.00

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Catalog #: 30012-1, 30012-2, 30012-3.

Product Description

Caspase-8 IETD-R110 Fluorometric HTS Assay Kit is specifically designed for HTS-based assays. The kit provides a homogenous assay system for fast and highly sensitive detection of caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells. The fluorogenic substrate (Ac-IETD)2-R110 contains two IETD tetrapeptides and is completely hydrolyzed by the enzyme in two successive steps. Cleavage of the first IETD peptide results in the monopeptide Ac-IETD-R110 intermediate, which has absorption and emission wavelengths similar to those of R110 (λExEm = 496/520 nm) but has only about 10% of the fluorescence of the latter. Hydrolysis of the second IETD peptide releases the dye R110, leading to a substantial fluorescence increase.

Note: While caspase-8 preferentially cleaves the consensus sequence IETD compared to other substrate sequences, other caspases such as caspase-3 also can cleave IETD efficiently. Overlapping caspase substrate recognition limits the usefulness of caspase substrate peptides for distinguishing between different caspase activities in cell lysates.

The assay kit includes Ac-IETD-CHO, which is a caspase-8 inhibitor and can be used as a negative control. Also, R110 is provided in the kit for generating a standard curve, which can be used for quantifying caspase-8 activity.

Kit Components:

  • Cell lysis/assay buffer
  • Enzyme substrate (Ac-IETD)2-R110
  • Enzyme inhibitor Ac-IETD-CHO
  • R110

Features:

  • HTS-compatible: Single-step homogenous assay specifically designed for HTS-based detection.
  • Fast: Fast enzyme kinetics.
  • Sensitive: The enzymatic reaction forms intensely green fluorescent rhodamine 110 (R110) product.

1) Biochemistry 38, 13906 (1999).

MSDS (PDF): MSDS 30012
Protocol (PDF): PI-30012

Multiple sizes

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life