CF™ dye goat anti-mouse IgG isotype-specific secondary antibody conjugates are labeled with our exceptionally bright, photostable CF™ dyes. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG1, IgG2a, or IgG2b. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG2a, IgG2b, IgG3), and human, bovine and rabbit serum proteins. CF™405S is a blue fluorescent dye with an absorption peak wavelength that nearly coincides with the 405 nm blue diode laser line. In addition, the emission peak wavelength of the dye well centers within the blue detection window of BD flow cytometers. As a result, flow cytometry analysis using CF™405S dye results in much brighter signal than that using Alexa Fluor® 405.Compatible with the NucleoCounter® NC-3000™.