CF488A Annexin V and 7-AAD Apoptosis Kit


Catalog #: 30060. Tags: , .

Product Description

Phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid protein with a high affinity for PS. Annexin V labeled with CF™488A (Abs/Em= 490/515 nm) can identify apoptotic cells in green by binding to PS exposed on the outer leaflet. Our CF™488A dye is superior to fluorescein/FITC as it is not affected by pH and has much better photostability. Moreover, 7-AAD emits in the far-red spectrum and can be well separated from CF™488A and other green emitting dyes using the 488nm line of a flow cytometer. 7-AAD is impermeant to live cells or apoptotic cells, but stains necrotic cells with red fluorescence (Abs/Em with DNA = 543/655 nm).

Compatible with the NucleoCounter® NC-3000™.


Flyer (PDF): FL-CF488A
MSDS (PDF): MSDS 30060
Protocol (PDF): PI-30060

100 assays

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Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life