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CF543 Tyramide

350.00

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Catalog #: 92172. Tags: , , .

Product Description

This  CF™543  tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal.  CF™543 has an excitation spectrum optimally matching the 543 nm laser line. Conjugates of the dye are much brighter than those of Alexa Fluor® 546.

Compatible with the NucleoCounter® NC-3000™.

1. Journal of Immunological Methods 125, 279-285 (1989).
2. Journal of Immunological Methods 137, 103-112 (1991).
3. Cytometry A. (2010), doi:10.1002/cyto.a.20970

Flyer (PDF): FL-CF543
MSDS (PDF): MSDS CFdye Tyramide
Protocol (PDF): PI-CF Dye or BiotinTyramide

0.5 mg

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life