In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3′- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF™640R TUNEL Assay Apoptosis Detection Kit employs dUTP conjugated to the exceptionally bright and photostable CF™640R far-red fluorescent dye (Ex/Em 642/662 nm), for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.
Compatible with the NucleoCounter® NC-3000™
1.Parrish and Xue (2006). Chromosoma 115: 89-97.; Gold et al. (1994). Lab Invest. 71(2):219-25.
2. Kapoor N, et al. (2013) Human Granuloma In Vitro Model, for TB Dormancy and Resuscitation.PLoS ONE 8(1): e53657. doi:10.1371/journal.pone.0053657
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