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CF640R Tyramide

$250.00

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Catalog #: 92175. Tags: , , .

Product Description

This  CF™640R  tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal.  CF™640R is a novel rhodamine-based dye spectrally very similar to Cy®5 and Alexa Fluor® 647. CF™640R is much brighter than Cy®5 and at least as bright as Alexa Fluor® 647 when excited at 633 or 635 nm. However, the most important advantage of CF™640R is its exceptional photostability. CF™640R is far more photostable than the cyanine-based dyes Cy®5 and Alexa Fluor® 647. It is also more photostable than Atto 647N, a dye that has been extensively used in single molecule-based imaging.

Compatible with the NucleoCounter® NC-3000™.

1. Journal of Immunological Methods 125, 279-285 (1989).
2. Journal of Immunological Methods 137, 103-112 (1991).
3. Cytometry A. (2010), doi:10.1002/cyto.a.20970

Flyer (PDF): FL-CF640R
MSDS (PDF): MSDS CFdye Tyramide
Protocol (PDF): PI-CF Dye or BiotinTyramide

0.5 mg

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life