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Coelenterazine (native)

74.20280.00

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Catalog #: 10110, 10110-1, 10110-2. Tag: .

Product Description

Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from ~0.1 uM to >100 uM (References 1-4). Renilla luciferase has been widely used as a reporter protein. Renilla luciferase/coelenterazine has also been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) (5) to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues (6-9). Biotium also offers Renilla Luciferase Assay Kits (30004) and Firefly & Renilla Dual Luciferase Assay Kits (30005). See the coelenterazine flyer under downloads for a comparison of coelenterazine analogs.

  • Yellow solid soluble in MeOH or EtOH
  • Store at -20°C and protect from light
  • C26H21N3O3
  • MW: 423
  • [55779-48-1]

For in-vivo imaging:

For reporter assay:

1. Diabetologia (2011) doi: 10.1007/s00125-011-2133-5 (2011).

2. PLoS ONE 6(2), e16873 (2011).
3. Chemistry and Biology 17, 1018 (2010).
4. Oncogene. 30(1), 77 (2011).
5. Cell Mol Neurobiol (2010) doi: 10.1007/s10571-010-9572-2
6. Molecular Imaging and Biology (2010) doi: 10.1007/s11307-010-0325-x
7. Molecular Cancer (2009) doi:10.1186/1476-4598-8-45
8. Analytical Biochemistry (2007) doi: 10.1016/j.ab.2007.08.038
9. The Plant Journal Vol. 48, 138-52 (2006).
10. Clinical Cancer Research 11, 4487-94 (2005).
11. Journal of Virology 79(14), 8909-19 (2005).
12. Cancer Research 64, 2113-9 (2004).
13. Cancer Research 64, 1323-30 (2004).
14. PNAS (2004) doi:10.1073/pnas.0304326101
15. Molecular Imaging 3 (1), 43-54 (2004).
16. Cancer Research 64, 6684-92 (2004).
17. Oncogene 22, 6865-72 (2003).
18. PNAS (2003) doi:10.1073/pnas.2435285100
19. Cancer Research 63, 1160-5 (2003).
20. Luminescence 2002 17(2), 77-115 (2002).
21. PNAS (2001) doi:10.1073/pnas.012611099
22. Protoplasma 218, 1-23 (2001).
23. Free Radic. Biol. Med. 28, 1232 (2000).
24. PNAS 96, 151 (1999).
25. Circ. Res. 84, 1203 (1999).
26. Meth. Cell Biol. 40, 305 (1994).
27. Cell Calcium, 14, 373 (1993).
28. J Cell Biol 115, 1259(1991).
29. Meth. Enzymol. 172, 164 (1989).

Flyer (PDF): Physical Properties of Coelenterazine Analogs
MSDS (PDF): MSDS 10110

Multiple sizes

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life