DiOC2(3) (3,3′-Diethyloxacarbocyanine, iodide) has been used for measuring membrane potentials in bacteria. The green fluorescent dye forms red fluorescent aggregates with increasing membrane potential. The spectral shifting phenomenon has been exploited to measure membrane potential ratiometrically.
- λEx/λEm (MeOH) = 482/497 nm
- Orange solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- MW: 460.31
1) Biophys J 56, 979 (1989).
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