DiSC2(5) (3,3′-Diethylthiadicarbocyanine, iodide) binds to DNA-DNA or PNA-DNA duplexes with a large blue-shift of the absorption spectrum. Binding of the dye to PNA-DNA hybrid, in particular, blue-shifts the dye absorption spectrum by as much as 115 nm, offering a visual method to detect nucleic acid hybridization (1,2).
- λAbs (pH 7.5 buffer) = 646 nm (free dye); ~586 nm (dsDNA); ~531 nm (PNA-DNA)
- Dark solid soluble in DMSO or MeOH
- Store at 4°C
- MW: 518.48
1. Nucleic Acid Res 30(2), 3 (2002).
2. J Am Chem Soc 121, 2686 (1999).
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