Firefly luciferase is widely used as a reporter gene for studying gene regulation and function, and for pharmaceutical screening (1). It is a very sensitive genetic reporter due to the lack of any endogenous luciferase activity in mammalian cells or tissues and the very nature of bioluminescence (2). Firefly luciferase is a 62,000 dalton protein which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation by oxygen into oxyluciferin with emission of light centered on 560 nm. As with many enzymes, firefly luciferase follows Michaelis-Menten kinetics, and as a result maximum light output is not achieved until the substrates and co-factor are present in large excess. When assayed under these conditions, light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. The Firefly Luciferase Assay Kit is designed for detection and quantification of firefly luciferase reporter enzyme from cultured cells in a simple, efficient and linear fashion. Firefly Luciferase Assay Kit (Lyophilized) performs comparably to Biotium’s original Firefly Luciferase Assay Kit (catalog no. 30003), but includes assay buffer in lyophilized format for more economical shipping and convenient storage at -20°C.
- 5X Firefly luciferase lysis buffer
- Firefly luciferase assay buffer (lyophilized)
Sensitivity and Linearity: Linear correlation between firefly luciferase enzyme levels and light output Convenience: Lyophilized buffer for economical shipping and convenient storage