Fluo-3, pentaammonium is membrane-impermeant, but can be loaded into cells via microinjection or scrape loading.
- Kd: 450 nM
- λEx/λEm (low or high [Ca2+]) = 506nm/526nm
- λµ (506 nm) = 86,000 M-1cm-1
- Orange red solid soluble in DMSO and water (pH >6)
- Store at 4°C and protect from light, especially when in solution
- MW: 854.7