Membrane-impermeant calcium indicator that can be loaded into cells via microinjection or scrape loading.
- Kd: 224 nM
- λEx/λEm (no Ca2+) = 363/512 nm; λEx/λEm (high Ca2+) = 335/505 nm
- λµ (363 nm, no Ca2+) = 27,000 M-1cm-1; λµ (335 nm, high Ca2+) = 35,000 M-1cm-1
- Light yellow solid soluble in DMSO
- Store at 4°C and protect from light, especially in solution
- MW: 832