70011.gif

JC-1 (chloride salt)

132.00

Clear
Catalog #: 70011. Tag: .

Product Description

JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (λ Em = 527 nm),while the J-aggregates have red fluorescence (λ Em = 590nm). Therefore, it is possible to use the fluorescence ratio technique to study mitochondrial membrane potentials. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green. It has also been used in high throughput drug screening applications. JC-1, iodide (#70014) is the original salt form of the dye. Biotium developed JC-1 chloride salt for applications where iodide ion is not desired. JC-1 chloride may also have the advantage of being slightly more water soluble than the iodide salt.

  • λµ (MeOH) = 190,000
  • Red solid soluble in DMSO
  • Store at 4°C and protect from light, especially in solution
  • C25H27Cl5N4
  • MW: 561.5
Compatible with the NucleoCounter® NC-3000™.

1. PNAS 88, 3671 (1991).
2. Biochemistry 30, 4480 (1991).

MSDS (PDF): MSDS 70011

5 mg

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life