Cell-biology111.gif

MTT Cell Viability Assay Kit

112.41

Clear
Catalog #: 30006.

Product Description

MTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using a standard colorimetric plate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and cytotoxicity caused by drugs and cytotoxic agents. Among all non-radioactive viability assays, MTT assay developed by Mossman is one of the most versatile and popular assays. MTT is a tetrazolium salt that is turned into a purple formazan product after reduction by mitochondrial enzymes that are only present in metabolically active live cells, not in dead cells. The amount of formazan product generated is proportional to the number of living cells in the sample. The formazan product can be solubilized and then photometrically quantified at 570 nm.

1. J Immunol Meth 174, 311 (1994).

2. J Immunol Meth 145, 199 (1991)

3. J Immunol Meth 131, 165 (1990).

4. Cancer Res 48, 598 (1988).

Flyer (PDF): Apoptosis, Necrosis, and Cell Viability Assays web
MSDS (PDF): MSDS 30006
Protocol (PDF): PI-30006

1,000 assays

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life