The fluorescence of NBD is highly dependent on the environment; it is virtually nonfluorescent in water but becomes strongly green fluorescent in hydrophobic media. NBD-ethylenediamine can be readily coupled to activated carboxy groups or sulfonyl chlorides.
- λEx/λEm (MeOH) = 466/535 nm
- Yellow solid soluble in DMSO or DMF
- Store at -20°C and protect from light
- MW: 223.19
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