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Phalloidin, Fluorescein conjugate

322.00

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Catalog #: 30.

Product Description

Phalloidin and phallacidin are collectively called phallotoxins, which are bicyclic peptides isolated from the deadly Amanita phalloides mushroom. Phallotoxins bind specifically to F-actin filaments with high affinity (1). Fluorescently labeled phallotoxins are very useful tool for investigating the distribution of F-actin. Labeled phallotoxins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and non-muscle cells from various species of plants and animals. Phallotoxins shift the F-actin monomer/polymer equilibrium toward the polymer, lowering the critical concentration for polymerization up to 30-fold (3,4). Phallotoxins also stabilize F-actin filaments, inhibiting depolymerization by cytochalasins, potassium iodide and elevated temperatures. Moreover, phallotoxin-labeled actin filaments remain functional; labeled glycerinated muscle fibers still contract, and labeled actin filaments still move on solid-phase myosin substrates (5,6). Fluorescent phallotoxins can also be used to quantify the amount of F-actin in cells (7,8). Fluorescein phalloidin stains F-actin with nanomolar affinity. Fluorescent phalloidin is typically used to stain fixed and permeabilized cells (1-3), but can also be loaded into live cells via cationic liposomes. Fluorescein phalloidin was one of the earlier fluorescent phallotoxins developed and is still widely used for visualizing F-actin. Also see our next generation CF™ dye phalloidin conjugates, which have superior brightness and photostability. Each vial (300 U) contains sufficient probe for staining ~300 samples with a staining volume of 200 uL per sample.

  • λAbsλEm (pH 8) = 496/516 nm
  • Yellow solid soluble in MeOH
  • Store at -20°C and protect from light
  • C56H60N10O15S2
  • MW: 1177

1. Wieland, T. in Phallotoxins, Springer-Verlag, New York (1986).
2. J Muscle Res Cell Motil 9, 370 (1988).
3. Methods Enzymol 85, 514 (1982).
4. Eur J Biochem 165, 125 (1987).
5. Nature 326, 805 (1987).
6. Proc Natl Acad Sci USA 83, 6272 (1986).
7. Blood 69, 945 (1987).
8. Anal Biochem 200, 199 (1992).
9. J Cell Biol 105, 1473 (1987).
10. Proc Natl Acad Sci USA 77, 980 (1980).

MSDS (PDF): MSDS 00030
Protocol (PDF): phalloidin

300 U

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