qPCR5.gif

PMA Enhancer for Gram Negative Bacteria, 5X Solution

59.61

Catalog #: 31038. Tag: .

Product Description

PMA™ is a high affinity photoreactive DNA binding dye developed by Biotium (40019). The dye is weakly fluorescent by itself but becomes highly fluorescent upon binding to nucleic acids. It preferentially binds to dsDNA with high affinity. Upon photolysis, the photoreactive azido group on the dye is converted to a highly reactive nitrene radical, which readily reacts with any hydrocarbon moiety at the binding site to form a stable covalent nitrogen-carbon bond, thus resulting in permanent DNA modification. The dye is cell membrane-impermeable and thus can be used to selectively modify DNA from dead cells with compromised membrane integrity, while leaving DNA from viable cells intact. PMA inhibits PCR amplification of modified DNA templates by a combination of removal of modified DNA during purification and inhibition of template amplification by DNA polymerases. Consequently the dye is useful in the selective detection of viable pathogenic cells by quantitative real-time PCR. PMA Enhancer for Gram Negative Bacteria was designed to improve PMA-mediated discrimination between live and dead gram-negative bacteria. PMA Enhancer is provided as a 5X solution, and is added to a sample before the addition of PMA. When a sequence from a gram-negative bacteria is amplified by PCR, samples pre-treated with Enhancer show a decrease in the signal from dead cells, with no change in the signal from live cells. Thus, PMA plus Enhancer is the optimal way to perform viability PCR on gram-negative bacteria. Note: PMA Enhancer is not intended for use when gram-positive bacteria are to be detected. PMA Enhancer may adversely affect the amplification of live cell DNA from gram-positive bacteria. For photoactivation of PMA or EMA dye, we recommend the use of Biotium’s PMA-Lite LED Photolysis Device (E90002), which is designed to conduct photolysis under controlled conditions. Also see our PMA Real-Time Bacterial Viability Kits.

 PMA diagram

Materials from Biotium are sold for research use only.

1. Nocker, A., et al. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J. Microbiol. Meth. 67(2), 310-320 (2006).

2. Nocker, A., et al. Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR. J. Microbiol. Meth. 70(2), 252-260 (2007).

3. Fittipaldi, M., et al. Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification. J. Microbiol. Meth. 91(2), 276-289 (2012).

Download the PMA reference list

Flyer (PDF): FL-PMA and PMA kits
MSDS (PDF): MSDS-31038
Protocol (PDF): PI-31038

16 mL

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life