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Renilla Luciferase Assay Kit

70.00595.00

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Catalog #: 30004-1, 30004-2, 30004-T. Tag: .

Product Description

Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. Recently, Renilla luciferase has been widely used in multiplex transcriptional reporter assays or as a normalizing transfection control for Firefly luciferase assay (1, 2). Renilla luciferase, a monomeric 36,000 Dalton protein, catalyzes coelenterazine oxidation by oxygen to produce light. The enzyme does not require post-translational modification for its activity, and may function as a genetic reporter immediately following translation. Coelenterazine native is the natural substrate for Renilla luciferase. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues. Biotium’s Renilla Luciferase Assay Kit is designed to provide a simple and sensitive method of detecting Renilla luciferase. Through the use of a specially designed buffer formulation, this assay kit yields reliable, linear results with minimal autoluminescence background and superior sensitivity.

Features:

  • Sensitivity and Linearity: Linear correlation between luciferase gene expression and light output
  • Convenience: Conveniently packaged substrate sizes permitting you to run a variable number of assays

1. Carcinogenesis 22 , 661 (2001).
2. J Biomol Screen 8 , 676 (2004).

MSDS (PDF): MSDS 30004
Protocol (PDF): PI-30004

Multiple sizes

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life