TMP-PEG3-amine, TFA salt


Catalog #: 91056.

Product Description

The trimethoprim-PEG3-amine (TMP-PEG3-amine) can be conjugated to various probes to make trimethoprim-based chemical tag (TMP-tag). The TMP-tag is based on the high affinity (KD ~ 1nM) binding of TMP to the E.coli dihydrofolate reductase (eDHFR). The target protein, fused to eDHFR via standard molecular cloning technology, is labeled by the TMP-probe in a non-covalent manner. The low affinity of TMP with mammalian DHFR (KD > 1µM) ensures minimal background. The TMP-tag has been demonstrated to enable protein visualization and manipulation both inside and on surface of mammalian cells.[1-4]

The TMP-PEG-amine can be used in combination with our Mix-n-Stain small ligand labeling kit to generate fluorescent chemical tags without purification for cell surface or intracellular staining.

[1] Miller, L.M et al (2005) Nat. Methods 2:255-257.

[2] Calloway, NT. et al (2007) ChemBioChem 8:767-774.

[3] Wombacher, R. et al (2010) Nat. Methods 7:717-719

[4] Long, MJ. et al (2011) J Am Chem Soc. 133:10006–10009

Size 1 mg

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