10-Acetyl-3,7-dihydroxyphenoxazine

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Product Description

10-Acetyl-3,7-dihydroxyphenoxazine (N-Acetyl-3,7-dihydroxyphenoxazine), which has been marketed under the name Amplex® Red, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H2O2 in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. High purity resorufin (10062) is also available from Biotium to be used as a standard.

  • λExEm (pH 9)= 571/585 nm (end product)
  • λµ= 54,000 (end product)
  • White to off-white solid soluble in DMSO or DMF
  • Store desiccated at 20°C and protect from light
  • C14H11CNO4
  • MW: 257.24
  • [119171-73-2]

Amplex Red is a registered trademark of Molecular Probes.

1. Biosensors and Bioelectronics (2010) doi:10.1016/j.bios.2010.07.126
2. Cancer Biotherapy and Radiopharmaceuticals 25(4), 439 (2010).
3. Anal Biochem 287, 196 (2000).
4. J Immunol Methods 202, 133 (1997).
5. Anal Biochem 253, 169 (1997).

MSDS (PDF): MSDS 10061

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life