AccuBlue Broad Range dsDNA Quantitation Kit with 9 DNA Standards



Product Description

The AccuBlue™ Broad Range dsDNA Quantitation Kit offers ultra-sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The kit provides a linear detection range from 2 – 2000 ng with sufficient reagents to perform 1000 assays based on a 96-well microplate format. The 1000 assay size kit includes AccuBlue™ Broad Range Quantitation Solution components and a set of 9 pre-diluted calf thymus dsDNA standards. The trial size kit includes one DNA standard solution with instructions for preparing a set of standards by serial dilution. AccuBlue™ Broad Range Quantitation Solution also is available separately (cat. no. 31009) for customers who wish to use their own DNA standards. The AccuBlue™ Broad Range assay is superior to the Quant-iT® Broad Range dsDNA and other detection kits by having a similar sensitivity but with more convenience and improved dynamic range. AccuBlue™ can be conveniently stored at 4°C, unlike the Quant-iT® reagents, which must be aliquoted for storage to avoid freeze-thaw cycles. The kit is compatible with fluorescence microplate readers and spectrofluorometers (λExEm: 350/460 nm). Biotium’s AccuLite 350 Mini Fluorometer (cat. no. E90000) includes a program for use with the AccuBlue™ Broad Range assay. Please also see our AccuBlue™ High Sensitivity dsDNA Quantitation Kit for quantitation of 0.2-100 ng dsDNA and our AccuClear™ Ultra High Sensitivity dsDNA Quantitation Kit for quantitation of 0.03-250 ng dsDNA. AccuBlue™ technology is covered by U.S. Patent No. 8,148,515.

1. PLoS ONE (2013), doi:10.1371/journal.pone.0080613
2. J Thromb Thrombolytic (2014), doi: 10.1007/s11239-014-1137-7
3. PLoS ONE (2015), doi:10.1371/journal.pone.0117792
4. Molecular Ecology (2015), doi: 10.1111/mec.13167

Flyer (PDF): AccuBlue & AccuClear
MSDS (PDF): MSDS 31007
Protocol (PDF): PI-31007

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life