AccuBlue High Sensitivity dsDNA Quantitation Kit with 8 DNA Standards



Product Description

The AccuBlue™  High Sensitivity dsDNA Quantitation Kit offers ultra-sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The assay provides a linear detection range from 0.2 – 100 ng with sufficient reagents to perform 1000 assays based on a 96-well microplate format. The 1000 assay size kit includes AccuBlue™ High Sensitivity Quantitation Solution components and a set of 8 pre-diluted calf thymus dsDNA standards. The trial size kit includes one DNA standard solution with instructions for preparing a set of standards by serial dilution. AccuBlue™ High Sensitivity Quantitation Solution also is available separately (Catalog #31008) for customers who wish to use their own DNA standards. The AccuBlue™ High Sensitivity assay is superior to PicoGreen® or Quant-iT® dsDNA detection kits by having a similar detection range but with more convenience and better safety. The high sensitivity reagent is impermeable to cell membranes, whereas PicoGreen® or the high sensitivity Quant-iT® DNA-binding dye readily crosses cell membranes. Moreover, the reagent is extremely stable. AccuBlue™ can be conveniently stored at 4°C, unlike PicoGreen® or Quant-iT® reagents, which must be aliquotted for storage to avoid multiple freeze-thaw cycles. Biotium’s AccuLite 470 Mini Fluorometer includes a program for use with the AccuBlue™ High Sensitivity assay. The assay (λExEm 485/530 nm) also is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers such as the Qubit® from Invitrogen. Please also see our AccuBlue™ Broad Range dsDNA Quantitation Kit for quantitation of 2-2000 ng and our AccuClear™ Ultra High Sensitivity dsDNA Quantitation Kit for quantitation of 0.03-250 ng DNA. AccuBlue™ technology is covered by U.S. Patent No. 8,148,515.

1. Conservation Genet Resour(2012), doi: 10.1007/s12686-011-9450-3
2. Parasites & Vectors (2012), doi:10.1186/1756-3305-5-59
3. Journal of Chromatography A (2010), doi:10.1016/j.chroma.2013.01.065
4. PLoS ONE (2013), doi:10.1371/journal.pone.0054743
5. Journal of Chromatography A (2013),
6. Journal of Biomechanics (2013),
7. Peer J (2013),
8. Journal of Biomechanics (2014),
9. Analytical Biochemistry (2014),
10. J. Bacteriol. (2014), doi:10.1128/JB.01493-14
11. The International Journal of Biochemistry & Cell Biology (2014), doi: 10.1016/j.biocel.2014.04.026
12. BMC Oral Health (2014), doi: 10.1186/1472-6831-14-157
13. Applications in Plant Sciences (2015),

Flyer (PDF): FL_AccuBlue AccuClear AccuLite
MSDS (PDF): MSDS 31006
Protocol (PDF): PI-31006

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Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

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