The AccuBlue™ NextGen dsDNA Quantitation Solution provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue™ NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA. With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue™ NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue™ NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.
The AccuBlue™ NextGen dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuBlue™ NextGen Dye make it especially well-suited for use with instruments with blue LED excitation sources. Because the AccuBlue™ NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see Table 1), assay volume, and pipet accuracy.
The AccuBlue™ NextGen dsDNA Quantitation Solution contains AccuBlue™ NextGen Dye, Enhancer, and assay buffer for 1000 96-well assays; the trial size kit contains enough reagents for 200 96-well assays. The Quantitation Solution does not include a DNA standard; see the AccuBlue™ NextGen dsDNA Quantitation Kit, which includes a calf thymus dsDNA standard.
- AccuBlue™ NextGen Dye, 400X
- AccuBlue™ NextGen Enhancer, 100X
- AccuBlue™ NextGen Buffer, 1X
- Linear range: 1-3000 pg dsDNA
- Green fluorescence (Ex/Em 460/507 nm)
- Unrivaled sensitivity
- Green fluorescent dye is a perfect match for blue LED excitation sources
- Green fluorescence detection compatible with fluorescence microplate readers, handheld fluorometers such as the AccuLite 470, and NanoDrop® fluorospectrophotometers.
|reader||assay format (assay volume)||lowest DNA detected|
|BMG CLARIOStar||96-well (200 uL)||1 pg|
|BMG CLARIOStar||384-well (50 uL)||0.6 pg|
|Biotek Synergy H1m||96-well (200 uL)||5 pg|
|SpectraMax Gemini XS||96-well (200 uL)||5 pg|
|AccuLite 470||PCR tube (200 uL)||5 pg|
Please also see related products for information on our other dsDNA Quantitation Kits such asAccuClear™ Ultra High Sensitivity dsDNA Quantitation Kit or Solution (.03-250 ng), AccuBlue™ High Sensitivity dsDNA Quantitation Kit or Solution (0.2-100 ng) and AccuBlue™ Broad Range dsDNA Quantiation Kit or Solution (2-2000 ng).
AccuBlue NextGen technology is covered by U.S. patents.