AccuBlue NextGen dsDNA Quantitation Solution


SKU: 31061, 31061-T Category:

Product Description

The AccuBlue™ NextGen dsDNA Quantitation Solution provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue™ NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA. With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue™ NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue™ NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.

The AccuBlue™ NextGen dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuBlue™ NextGen Dye make it especially well-suited for use with instruments with blue LED excitation sources. Because the AccuBlue™ NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see Table 1), assay volume, and pipet accuracy.

The AccuBlue™ NextGen dsDNA Quantitation Solution contains AccuBlue™ NextGen Dye, Enhancer, and assay buffer for 1000 96-well assays; the trial size kit contains enough reagents for 200 96-well assays. The Quantitation Solution does not include a DNA standard; see the AccuBlue™ NextGen dsDNA Quantitation Kit, which includes a calf thymus dsDNA standard.

Kit components

  • AccuBlue™ NextGen Dye, 400X
  • AccuBlue™ NextGen Enhancer, 100X
  • AccuBlue™ NextGen Buffer, 1X


  • Linear range: 1-3000 pg dsDNA
  • Green fluorescence (Ex/Em 460/507 nm)
  • Unrivaled sensitivity
  • Green fluorescent dye is a perfect match for blue LED excitation sources
  • Green fluorescence detection compatible with fluorescence microplate readers, handheld fluorometers such as the AccuLite 470, and  NanoDrop® fluorospectrophotometers.

Table 1

reader assay format (assay volume) lowest DNA detected
BMG CLARIOStar 96-well (200 uL) 1 pg
BMG CLARIOStar 384-well (50 uL) 0.6 pg
Biotek Synergy H1m 96-well (200 uL) 5 pg
SpectraMax Gemini XS 96-well (200 uL) 5 pg
AccuLite 470 PCR tube (200 uL) 5 pg

Please also see related products for information on our other dsDNA Quantitation Kits such asAccuClear™ Ultra High Sensitivity dsDNA Quantitation Kit or Solution (.03-250 ng), AccuBlue™ High Sensitivity dsDNA Quantitation Kit or Solution (0.2-100 ng) and AccuBlue™ Broad Range dsDNA Quantiation Kit or Solution (2-2000 ng).

AccuBlue NextGen technology is covered by U.S. patents.


Flyer (PDF): AccuBlue NextGen

MSDS (PDF): MSDS 31061

Protocol (PDF): PI-31061

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life