Bacterial Viability and Gram Stain Kit



Product Description

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria. Fluorescently labeled wheat germ agglutinin binds specifically to the N-acetylglucosamine of the peptidoglycan layer of gram-positive bacteria1. The bacterial viability component of the assay is based on the integrity of the cell membrane. EthD-III is a nucleic acid binding dye that is membrane impermeable, which selectively stains cells with compromised plasma membranes. All cells stain blue with the membrane permeable DNA dye DAPI. One kit is sufficient 200 assays when used according to the kit protocol. The Bacterial Viability and Gram Stain Kit has been tested on the following bacterial species: Bacillus subtilis subsp. subtilis, Escherichia coli, Micrococcus luteus, Pseudomonas fluorescens, and Staphylococcus epidermidis.


MSDS (PDF): MSDS 32001
Protocol (PDF): PI-32001

1 kit

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life