BCECF, free acid


SKU: 51010 Categories: , ,

Product Description

BCECF (2′,7′ –bis-(Carboxyethyl)-5-(and-6)-carboxyfluorescein) is the most widely used fluorescent pH sensor. Because its pKa (6.97) is close to physiological pH, BCECF can detect cytosolic pH change with high sensitivity. At low pH, the dye is weakly fluorescent but becomes more fluorescent with increasing pH. The excitation spectrum of the dye undergoes a slight shift during pH change, while the wavelength of the emission maximum remains unchanged.

The pH is determined ratiometrically by the relative fluorescent intensities at 535 nm when the dye is excited at 439 nm and505 nm respectively. BCECF, acid is membrane-impermeant and therefore can be loaded into cells via microinjection or scrape loading.

Also see the membrane permeant AM ester form of BCECF (51011).

  • λExEm = 508nm530nm
  • Yellow orange solid soluble in DMSO
  • Store at 4°C and protect from light, especially in solution
  • MW: 520.5
  • [85138-49-4]

1. J Immunol Meth 108,
255 (1988).
2. Biochem J 304, 751 (1994).
3. J Immunol Meth 172, 255 (1994).

MSDS (PDF): MSDS 51010

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life