Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number ’11’ is the number of atoms in the linker between biotin and dUTP. Biotium also offers biotin-16-dUTP (40022, 40022-1) and biotin-20-dUTP (catalog nos. 40030, 40030-1). The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, a longer linker should facilitate interaction between biotin and avidin or streptavidin.
- Lyophilized solid more suitable for long term storage. The product contains lyophilized TE buffer.
- Store at -20°C
- MW: 886.5
Biotin-11-dUTP is also available as a 1 mM solution in TE buffer (40029).