Biotin-16-dUTP, 1 mM solution


Product Description

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3′ end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies. The number ’16’ is the number of atoms in the linker between biotin and dUTP. Biotium also offers biotin-11-dUTP (catalog no. 40029) and biotin-20-dUTP (catalog no. 40030). The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, a longer linker should facilitate interaction between biotin and avidin or streptavidin.

  • 1 mM solution in pH 7.5 Tris-HCl buffer
  • Store at -20°C
  • C32H48N7O18P3Sli4
  • MW: 971.5

Biotin-16-dUTP is also available as a lyophilized solid (catalog no. 40022-1).

1. Anal Biochem 193, 2 (1991).
2. J Cell Biol 119, 493 (1992).
3. NeuroReport, 7, 61 (1995).
4. Biotechniques 19, 800 (1995).
5. Journal of Virological Methods (2013),
6. The Open Genomics Journal 5, 18-29 (2012)

Flyer (PDF): Nucleotide Conjugates
MSDS (PDF): MSDS 40022

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life