Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM, a nonfluorescent molecule itself, is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent Calcein. The fluorescent Calcein is retained in the cytoplasm in live cells. Calcein AM has been served as an excellent tool for the studies of cell membrane integrity and for long-term cell tracing.
Calcein AM Cell Viability Assay Kit is designed to quantify live cell numbers based on the presence of their cytoplasmic membrane integrity. It is a true end-point assay for cell viability. The fluorescent signal is monitored using 485 nm excitation wavelength and 530 nm emission wavelength. The fluorescent signal generated from the assay is proportional to the number of living cells in the sample.
- Calcein AM in anhydrous DMSO
- True end-point viability assay: Detect only cells with uncompromised cytoplasmic membrane integrity
- Simple & Fast: 30-min assay.