Calcein AM Cell Viability Assay Kit



Product Description

Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM, a nonfluorescent molecule itself, is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent Calcein. The fluorescent Calcein is retained in the cytoplasm in live cells. Calcein AM has been served as an excellent tool for the studies of cell membrane integrity and for long-term cell tracing.

Calcein AM Cell Viability Assay Kit is designed to quantify live cell numbers based on the presence of their cytoplasmic membrane integrity. It is a true end-point assay for cell viability. The fluorescent signal is monitored using 485 nm excitation wavelength and 530 nm emission wavelength. The fluorescent signal generated from the assay is proportional to the number of living cells in the sample.

Kit Components:

  • Calcein AM in anhydrous DMSO


  • True end-point viability assay: Detect only cells with uncompromised cytoplasmic membrane integrity
  • Simple & Fast: 30-min assay.
Compatible with the NucleoCounter® NC-3000™.

1. J Neurosci Methods. 50, 205 (1993).
2. J Immunol Methods 163,181 (1993).
3. Cancer Lett. 87, 199 (1994).
4. J Immunol Methods 178, 41 (1995).

MSDS (PDF): MSDS 30026
Protocol (PDF): PI-30026

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life