CellBrite™ Green Cytoplasmic Membrane Dye is designed to label cell cytoplasmic membranes with green fluorescence (Abs/Em = 484/501 nm). The labeling is stable and nontoxic, suitable for long-term tracking of cells. The kit utilizes the highly green fluorescent and lipophilic membrane dye Neuro-DiO (catalog no. 60015) pre-dissolved in an optimized staining solution. Neuro-DiO was developed by Biotium as an alternative to the traditional membrane dye DiO to overcome the low solubility and aggregation problems of the latter. Similar to other lipophilic carbocyanine dyes, Neuro-DiO has two long C18 hydrocarbon chains that insert into the lipid bilayer, resulting in stable labeling which is resistant to intercellular dye transfer (1). Moreover, Neuro-DiO is very photostable, which should facilitate microscopic studies of the stained cells. Cell staining is accomplished by adding the supplied dye solution directly to normal culture media, followed by a brief incubation period. By combining multiple CellBrite™ Cytoplasmic Membrane Stains, one can label multiple cell populations with different colors for studies of cell-cell interactions.
- Neuro-DiO cell-labeling solution
- Simple: Cell staining by direct addition of the supplied dye solution to normal culture media
- Stable Labeling: Highly lipophilic cyanine dye ensuring no dye transfer between cells
- Bright, Photostable and Nontoxic: Carbocyanine dye with high fluorescent quantum yield, high photostability and relatively low toxicity.