Cellular senescence is a state of permanent cell-cycle arrest induced by cell age, stress, or certain cytotoxic drugs. Senescent cells show enlarged, flattened morphology and upregulation of beta-galactosidase activity. This kit is designed for beta-galactosidase staining using the blue chromogenic substrate X-gal under buffer conditions that allow selective staining of senescence-associated beta-galactosidase activity (SA-β-gal). X-gal staining of SA-β-gal activity has been reported for fresh-frozen tissue cryosections, but is not compatible with formalin-fixed paraffin-embedded (FFPE) tissue samples.
The kit contains enough reagents for 100 assays in 35 mm dishes with 2 mL staining volume, the volume can be scaled as needed depending on the size of the specimen or culture dish.
30031A: X-gal 2 x 100 mg
99803: 10X Fixative Solution 20 mL
99804: Cellular Senescence Staining Buffer 200 mL