This CF™405L tyramide conjugate is used for tyramide signal amplification (TSA)1,2,3, which is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to generate high-density labeling of a target protein or nucleic acid in situ. A tyramide conjugate is incubated in the presence of HRP (often bound to a secondary antibody), which catalyzes the binding of the tyramide (and associated label) to adjacent tyrosines for enhanced fluorescent signal. CF™405L is an orange fluorescent dye spectrally similar to Pacific Orange dye.
1. Journal of Immunological Methods 125, 279-285 (1989).
2. Journal of Immunological Methods 137, 103-112 (1991).
3. Cytometry A. (2010), doi:10.1002/cyto.a.20970
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