CF™ dye goat anti-mouse IgG isotype-specific secondary antibody conjugates are labeled with our exceptionally bright, photostable CF™ dyes. The conjugates are prepared from affinity-purified antibodies that react with Fc portion of the heavy chain of mouse IgG1, IgG2a, or IgG2b. To minimize cross reactivity, the antibodies are cross-adsorbed against other mouse IgG subclasses (IgG2a, IgG2b, IgG3), and human, bovine and rabbit serum proteins. Orange fluorescent CF™543 has excitation/emission maxima at 541/560 nm, and produces significantly brighter antibody conjugates than the spectrally similar dye Alexa Fluor® 546. Alexa Fluor is a registered trademark of Molecular Probes Inc.