DiSC2(5) (3,3′-Diethylthiadicarbocyanine, iodide) binds to DNA-DNA or PNA-DNA duplexes with a large blue-shift of the absorption spectrum. Binding of the dye to PNA-DNA hybrid, in particular, blue-shifts the dye absorption spectrum by as much as 115 nm, offering a visual method to detect nucleic acid hybridization (1,2).
- λAbs (pH 7.5 buffer) = 646 nm (free dye); ~586 nm (dsDNA); ~531 nm (PNA-DNA)
- Dark solid soluble in DMSO or MeOH
- Store at 4°C
- MW: 518.48
1. Nucleic Acid Res 30(2), 3 (2002).
2. J Am Chem Soc 121, 2686 (1999).
Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.
Expedited shipment may be requested at time of checkout.
Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.
More information about product handling and shelf life