Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate cell loading. Fura-2, AM ester itself does not bind Ca2+, but it is readily hydrolyzed to Fura-2 by endogenous esterases once it enters cells.
- Light yellow solid soluble in DMSO
- Store at -20°C and protect from light, especially in solution
- MW: 1001.9
For more information on Fura-2, see 50030.