Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258. Biotium also offers Hoechst 33342 in solution (catalog no. 40046).
- λEx/λEm (with DNA) = 350/461 nm
- Pale yellow solid soluble in water up to 2% (w/v)
- Store at 4°C and protect from light
- MW: 615.98
Compatible with the NucleoCounter® NC-3000™
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