Product Description
Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis. Live-or-Dye™594/614 has also been validated for use in live/dead cell discrimination in fluorescence microscopy.
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells.
The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
Kit contents:
50 assay trial size:
- 1 vial lyophilized reactive dye (50 labeling reactions)
- Anhydrous DMSO for dye reconstitution
200 assay size:
- 4 vials lyophilized reactive dye (50 labeling reactions per vial)
- Anhydrous DMSO for dye reconstitution
λEx/λEm = 561/624 nm 
Biotium offers a selection of eight different Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis (see below). [table “9” not found /]
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