Lumitein Protein Gel Stain 100X

$40.00$500.00

Clear
SKU: 21002, 21002-1, 21002-2 Categories: ,

Product Description

Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels, but that can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility with common instruments and downstream analysis. Lumitein™ protein gel stain is supplied as a highly concentrated 100X solution, which provides convenience for shipping, handling and storage. More importantly, the concentrated solution is economical because of reduced manufacturing and shipping costs. Lumitein is also available as a convenient ready-to-use 1X staining solution (catalog no. 21001).

Features

    • Highly Sensitive: At least as sensitive as silver stain by detecting < 1 ng protein.
    • Simple & Fast: Fixation and staining in a single 30-90 minute step, followed by a simple rinse in water.
    • Very Low Background
    • Instrument Compatibility: Image with 300 nm UV gel box (EtBr filter), Dark Reader, or a laser scanner.
    • Wide Linear Detection Range: At least three orders of magnitude.
    • Compatible with Downstream Analysis: Compatible with MS and sequencing.
    • Highly Stable: Both 100X and 1X solutions are stable at room temperature.
    • Economical: 100X concentrate with reduced manufacturing and shipping costs

 

 

 

Figure 1. Two-fold serial dilutions of protein marker were separated via SDS-PAGE and then stained with Lumitein. Imaged with a GE Typhoon Trio using 532 nm excitation and 610BP30 emission filter. Figure 2. 2-D gel of human liver protein lysate stained with Lumitein. The three circled spots were picked for MS analysis by Applied Biomics, Inc. (Hayward, CA), confirming that Lumitein staining is fully compatible with MS analysis.

 

Lumitein vs. Coomassie

Lumitein

 

Coomassie

 

Figure 3. PAGE gels containing electrophoretically separated protein marker (loaded in two-fold serial dilution from left to right) were stained with Lumitein total protein gel stain (left) and Coomassie Blue (right), respectively. Lumitein-stained gel was imaged using a UV box equipped with EtBr filter (UVP) while the Coomassie-stained gel was imaged using a white light converter (UVP).

1. Meth Mol Biol. 869, 621 (2012).
2. Appl Environ Microbiol (2013) doi:10.1128/AEM.01478-13
3. J Mol Biol (2012) doi:10.1016/j.jmb.2012.10.018
4. Environ Microbiol 12, 2190 (2010).
5. Can J Microbiol 55, 304 (2009).
6. Res Microbiol 160, 401 (2009).
7. Org. Biomol. Chem. (2014) doi: 10.1039/C4OB00622D
8. Cell Reports (2014), http://dx.doi.org/10.1016/j.celrep.2014.03.072
9. PNAS (2014), www.pnas.org/cgi/doi/10.1073/pnas.1407862111
10. PLoS ONE (2014), doi:10.1371/journal.pone.0112874
11. International Journal of Food Microbiology (2015), http://dx.doi.org/10.1016/j.ijfoodmicro.2014.12.012
12. BMC Microbiology (2015), doi: 10.1186/s12866-015-0387-7

Flyer (PDF): Lumitein
MSDS (PDF): MSDS 21002
Protocol (PDF): PI-21002

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life