MCB Glutathione Detection Kit



Product Description

Diminished cellular glutathione (GSH) level occurs at the early stage of mitochondrion-associated apoptosis pathway due to GSH efflux. GSH depletion further leads to cytochrome c release and caspase 3 induction (1,2). MCB Glutathione Detection Kit utilizes a thiol-reactive dye monochlorobimane (MCB), which is essentially nonfluorescent until it reacts with a thiol to form a blue fluorescent product (λAbsEm = 380/461 nm). By incubating cell lysate with MCB, the intensity of the fluorescent signal generated from the assay reflects the amount of GSH present in the cells. Fluorescence can easily be detected using a fluorometer or 96-well fluorometric plate reader with approximately 1 hour assay time.

Kit Components:

  • Cell lysis buffer
  • Monochlorobimane (MCB)
  • GST positive control
Compatible with the NucleoCounter® NC-3000™.

1. FASEB J 12 (6), 479 (1998).
2. Biochem Soc Trans 28, 56 (2000).

MSDS: MSDS 30019
Protocol (PDF): PI-30019

100 assays

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life