Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step. The ligand to be labeled must contain an aliphatic amine group that is biologically inert. The amino group will form a covalent linkage with the provided reactive CF dyes. For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligand derivatives that have an aliphatic amine. For more information, please download the Mix-n-Stain™ small ligand labeling kit flyer and the product information sheet for this product. This kit is for labeling the small ligand with red fluorescent CF™568 for surface staining of live cells. CF™568 has an excitation spectrum optimally matching the 568 nm line of the Ar-Kr mixed-gas laser. Protein conjugates of the dye are much brighter and more photostable than those of Alexa Fluor® 568. Mix-n-Stain™ small ligand labeling kits for cell surface labeling are available in your choice of 10 CF™ dye colors for labeling ligands for cell surface targets, and three CF™ dye colors for ligands that bind intracellular targets. Also see our Mix-n-Stain™ Antibody Labeling Kits and other Reactive Dyes and Biotin Reagents.
|Dye||Catalog no.||Ex (nm)||Em (nm)||Staining|