MUG (4-MU-glcUA; 4-Methylumbelliferyl-β-D-glucuronic acid dihydrate), also known as MUGlcU, is a fluorogenic β-glucuronidase substrate that releases the blue fluorescent 4-methyl-7-hydroxycoumarinon enzymatic hydrolysis. The substrate is commonly used for identifying E. coli contamination and for detecting marker GUS gene expression in plants with high sensitivity.
- λ Ex/λ Em= 360/450 nm (end product)
- White solid soluble in DMSO
- Store desiccated at -20°C and protect from light
- MW: 388.3
1. BioTechniques 8, 39 (1990).
2. Plant Mol. Biol. Rep. 5, 387 (1987).
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