This kit contains the NucView™488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry. The bi-functional NucView™488 Caspase-3 Substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView™633 is a new far-red fluorescent mitochondrial dye, which diffuses across the plasma membrane and accumulates in the mitochondria. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView™633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap. Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes. See our full selection of NucView™ substrates and kits. NucView™ enzyme substrate technology is covered by U.S. Patents.