NucView™ 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time. Visit this Product Video page to see a video of NucView™ 488 in action.
Unlike conventional caspase assays, NucView™ 488 Caspase-3 Substrate detects caspase-3/7 activity within individual intact cells without inhibiting caspase activity. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3/7. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucView™ 488 Caspase-3 Substrate is bi-functional, allowing detection of intracellular caspase-3/7 activity and visualization of changes in nuclear morphology during apoptosis.
The fluorescent staining produced in response to caspase-3/7 activity is compatible with subsequent fixation and permeabilization for immunostaining. NucView™ 488 Caspase-3 Substrate, is offered as a 1 mM solution in DMSO (catalog no. 10402) or PBS (phosphate-buffered saline). The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity. In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView 488 staining.
In addition to stand-alone substrate stock solutions, we offer NucView™ 488 assay kits with detailed application protocols. Biotium also offers the blue fluorescent NucView™ 405 Caspase-3 Substrate for use with the 405 nm laser line. Download the NucView™ flyer for more information. NucView™ enzyme substrate technology is covered by U.S. patents.Compatible with the NucleoCounter® NC-3000™.