NucView 488 Caspase-3 Enzyme Substrate, 1 mM in PBS



Product Description

NucView™ 488 Caspase-3 Substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3/7 activity within live cells in real time. Visit this Product Video page to see a video of NucView™ 488 in action.

Unlike conventional caspase assays, NucView™ 488 Caspase-3 Substrate detects caspase-3/7 activity within individual intact cells without inhibiting caspase activity. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3/7. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucView™ 488 Caspase-3 Substrate is bi-functional, allowing detection of intracellular caspase-3/7 activity and visualization of changes in nuclear morphology during apoptosis.

The fluorescent staining produced in response to caspase-3/7 activity is compatible with subsequent fixation and permeabilization for immunostaining. NucView™ 488 Caspase-3 Substrate, is offered as a 1 mM solution in DMSO (catalog no. 10402) or PBS (phosphate-buffered saline). The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity. In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView 488 staining.

In addition to stand-alone substrate stock solutions, we offer NucView™ 488 assay kits with detailed application protocols. Biotium also offers the blue fluorescent NucView™ 405 Caspase-3 Substrate for use with the 405 nm laser line. Download the NucView™ flyer for more information. NucView™ enzyme substrate technology is covered by U.S. patents.

Compatible with the NucleoCounter® NC-3000™.

Cen, et al. DEVD-NucView488: a novel class of enzyme substrates for real-time detection of caspase-3 activity in live cells. The FASEB Journal published online February 8, 2008.

Download a full list of references: Validated cell lines for NucView 488 with references

Flyer (PDF): NucView flyer
MSDS (PDF): MSDS 10403
Protocol (PDF): PI-10402, 10403

(PDF): Validated cell lines for NucView 488 with references

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

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Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

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